Device

Part:BBa_J100202

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2014-11-21)


Intermediate for IndClone Red

We are building actClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 805
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 805
    Illegal AgeI site found at 678
    Illegal AgeI site found at 790
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aIntermediate for IndClone Red